publications
Article
Association of FokI polymorphism of vitamin D receptor with urothelial bladder cancer in Tunisians: role of tobacco smoking and plasma vitamin D concentration |
Ben Fradj Mohmed Kacem, FEKI Moncef, JEMAA Riadh, KAABACHI Naziha, KALLEL Amani, 2015 |
TUMOR BIOLOGY, 6, 10.1007/s13277-015-4496-6, Novembre 2015 |
Résumé
The aim of the study was to test whether the VDR
FokI polymorphism is associated with the risk of urothelial
bladder cancer (UBC) in Tunisians. The study included 200
unrelated patients with UBC and 200 healthy controls.
Genotyping of the VDR FokI polymorphism was determined
by PCR-RFLP method. Plasma 25-hydroxyvitamin D concentrations
were measured by immunoassay. Binary logistic regression
model was applied to test how the association of
VDR FokI polymorphism is independent of potential confounding
factors. Genotype distribution (FF, 45 vs. 55 %; Ff,
52.1 vs. 47.9 %, and ff, 12 vs. 5.5 %, respectively) and allele
frequencies (F, 66.5 vs. 74.8 % and f, 33.5 vs. 25.2 %, respectively)
were significantly different between UBC patients and
controls. The Bff^ genotype [OR (95 % CI), 2.66 (1.24–5.73);
p=0.012] and Bf^ allele [1.49 (1.09–2.02); p=0.010] were associated
with increased risk of UBC. The association remained
significant in multivariate analysis. Stratified analyses showed
that VDR FokI polymorphism is only associated with UBC risk
in ever-smokers, subjects exposed to chemical carcinogens and
those with plasma 25-hydroxyvitamin D over 12 μg/L. The Bf^
allele of VDR FokI polymorphism is associated with a higher
risk of UBC in Tunisians, es
