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Emergence of Carbapenem-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Clinical Isolates Collected from Some Libyan Hospitals

MATHLOUTHI Najla, AREIG Zaynab, EL SALABI Allaaeddin, BEN SLAMA Karim, AL BAYSSARI Charbel, BEN GWIERIF Salha, CHOUCHANI Chedly, BAKOUR Sofiane, ZORGANI Abdulaziz, ROLAIN Jean-Marc, 2015

Microbial Drug Resistance, 21, 3, 335-341, Juin 2015

Résumé

The aim of the present study was to investigate the molecular mechanism of carbapenem resistance in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates recovered from Libyan hospitals between April 2013 and April 2014. In total, 49 strains (24 P. aeruginosa and 25 A. baumannii) were isolated, including 21 P. aeruginosa and 22 A. baumannii isolates (87.75%) resistant to imipenem (minimum inhibitory concentrations ‡ 16 mg/ml). The blaVIM-2 gene was detected in 19 P. aeruginosa isolates. All imipenemresistant P. aeruginosa isolates showed the presence of OprD mutations. Acquired OXA-carbapenemaseencoding genes were present in all A. baumannii isolates: blaOXA-23 (n = 19) and blaOXA-24 (n = 3). Finally, a total of 13 and 17 different sequence types were assigned to the 21 P. aeruginosa and the 22 A. baumannii carbapenem-resistant isolates, respectively. This study is the first report describing imipenem-resistant P. aeruginosa and A. baumannii isolated from patients in Libya. We report the first case of co-occurrence of blaVIM-2 with oprD porin loss in identical isolates of P. aeruginosa in Libya and demonstrate that these oprD mutations can be used as a tool to study the clonality in P. aeruginosa isolates. We also report the first identi- fication of multidrug-resistant A. baumannii isolates harboring blaOXA-23-like, blaOXA-24-like, and blaOXA-48-like genes in Libya.

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