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Article

Description of Geodermatophilus bullaregiensis sp. nov.

HEZBRI Karima, GHODHBANE-GTARI Faten, MONTERO-CALASANZ Maria Del Carmen , SGHAIER Haitham, ROHDE Manfred, SCHUMANN Peter, KLENK Hans-Peter, GTARI Maher, 2015

Antonie van Leeuwenhoek, 108, 2, 415-425, Août 2015

Résumé

The taxonomic position of an aerobic actinobacterial strain, BMG841T, isolated from the Bulla Regia monument (Tunisia) and exhibiting a high resistance to gamma-radiation (D10 *9 kGy) was determined using polyphasic approach. The optimal growth range was found to be 25–35 _C at pH of 7.0–8.5. The strain was observed to form black dry colonies. Chemotaxonomic characteristics of the isolate showed a cell wall type III, with galactose and glucose as diagnostic sugars; phosphatidylcholine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid as main polar lipids; and MK- 9(H4) as the predominant menaquinone. The major cellular fatty acids were identified as iso-C16:0 and iso-C15:0. Phylogenetic analysis indicated that strain BMG841T represents a novel member of the genus Geodermatophilus with high 16S rRNA gene sequence identity with Geodermatophilus saharensis (98.28 %). Based on phylogenetic and phenotypic analysis, strain BMG841T is proposed as the type strain (=DSM 46841T = CECT 8821T) of a novel species, Geodermatophilus bullaregiensis.