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Article

Geodermatophilus sabuli sp. nov., a γ-radiation-resistant actinobacterium isolated from desert limestone

HEZBRI Karima, GHODHBANE-GTARI Faten, MONTERO-CALASANZ Maria Del Carmen , SGHAIER Haitham, ROHDE Manfred, SCHUMANN Peter, KLENK Hans-Peter, GTARI Maher, 2015

International Journal of Systematic and Evolutionary Microbiology, 65, 10, 3365-3372, Octobre 2015

Résumé

A novel c-radiation-resistant and Gram-staining-positive actinobacterium designated BMG 8133T < was isolated from a limestone collected in the Sahara desert of Tunisia. The strain produced dry, pale-pink colonies with an optimum growth at 35–40 8C and pH 6.5–8.0. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and one unspecified glycolipid. MK-9(H4) was the dominant menaquinone. Galactose and glucose were detected as diagnostic sugars. The major cellular fatty acids were branched-chain saturated acids iso-C16 : 0 and iso-C15 : 0. The DNA G+C content of the novel strain was 74.5 %. The 16S rRNA gene sequence showed highest sequence identity with Geodermatophilus ruber (98.3 %). Based on phenotypic results and 16S rRNA gene sequence analysis, strain BMG 8133T is proposed to represent a novel species, Geodermatophilus sabuli sp. nov. The type strain is BMG 8133T (5DSM 46844T5CECT 8820T).