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Article

Description of gamma radiation‑resistant Geodermatophilus dictyosporus sp. nov. to accommodate the not validly named Geodermatophilus obscurus subsp. dictyosporus (Luedemann, 1968)

MONTERO-CALASANZ Maria Del Carmen , HEZBRI Karima, GÖKER Markus, SGHAIER Haitham, ROHDE Manfred, SPRÖER Cathrin, SCHUMANN Peter, KLENK Hans-Peter, 2015

Extremophiles, 19, 1, 77-85, Janvier 2015

Résumé

A gamma radiation-resistant, Gram reactionpositive, aerobic and chemoorganotrophic actinobacterium, initially designated Geodermatophilus obscurus subsp. Dictyosporus G-5T, was not validly named at the time of initial publication (1968). G-5T formed black-colored colonies on GYM agar. The optimal growth range was 25–35 °C, at pH 6.5–9.5 and in the absence of NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G +C content of the strain was 75.3 mol %. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main polar lipids  were phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and one unspecified glycolipid; MK-9(H4) was the dominant menaquinone and galactose was detected as a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids, iso-C16:0 and iso-C15:0. The 16S rRNA gene showed 94.8–98.4 % sequence identity with the members of the genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G-5T is proposed to represent a novel species, Geodermatophilus dictyosporus and the type strain is G-5T (=DSM 43161T = CCUG 62970T = MTCC 11558T = ATCC 25080T = CBS 234.69T = IFO 13317T = KCC A-0154T = NBRC 13317T). The INSDC accession number is HF970584.