publications
Article
Occurrence and antimicrobial susceptibility pattern of ESBL-producers among Gram-negative bacteria isolated from burn unit at the Al Shifa hospital in Gaza, Palestine: A short original article. |
TAYH Ghassan, AL LAHAM Nahed, ELMANAMA Abdelraouf, BEN SLAMA Karim, 2016 |
The International Arabic Journal of Antimicrobial Agents, 5, 3, Janvier 2016 |
Résumé
Background: Bacterial infection can be an important cause of death in burn cases. The emergence of antimicrobial resistant pathogens associated with extended spectrum beta-lactamases (ESBLs) is lead-ing to inappropriate/or treatment failure and increased morbidity and mortality.
Objective: The purpose of this study was to determine the antimicro-bial susceptibility pattern with the identification of ESB -producers among pathogens isolated from burn patients at the Al-Shifa burn unit in Gaza, Palestine.
Methods: A total of 40 gram-negative bacterial isolates were recov-ered from burn wound patients between August 2012 and December 2012. Antibiotic susceptibility profiles of the identified isolates were determined by the standard disc diffusion method according to CLSI guidelines. The ESBL detection was screened by using double disc diffusion method.
Results: Pseudomonas aeruginosa was the predominant isolate 15 (37.5%) followed by Klebsiella pneumoniae 10 (25%), Escherichia coli 9 (22.5%), Enterobacter cloacae 4 (10%) and Acinetobacter baumannii2 (5%). ESBL was detected in 37.5% of isolates. Most of ESBL-producing isolates were susceptible to meropenem and imipenem (80% and 73%, respectively). However, ESBL producing isolates showed 100% resistance against cefotaxime and ampicillin. The resistance rate towards sulfamethoxazole/trimethoprim, tetracycline, cefepime, tobramycin and aztreonam was 76.9%, 69.2%, 66.7%, 60% and 60%, respectively. A. baumannii isolates were resistance to all tested antibiotics.
Conclusion: P. aeruginosa is the most common bacteria isolated from burn wound of patients at the Al-Shifa burn unit. The frequency of ESBL-producers among gram-negative bacteria was high, and most of these isolates except A. baumannii were still highly susceptible to carbapenems including imipenem and meropenem.
