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Article

Phylogeny of Mediterranean Lathyrus species using Inter Simple Sequence Repeats markers.

CHTOUROU-GHORBEL Nidhal, GHORBEL Mouna, MARGHALI Sonia, TRIFI Neila, 2014

Acta Botanica Gallica, 161 (1), 2014, 91-98, Février 2014

Résumé

The genus Lathyrus constitutes an important phytogenetic patrimony as a source of traditional foodstuffs inTunisia. An Inter-Simple Sequence Repeats (ISSR) technique was employed to assess genetic diversity and relationshipsof seven Mediterranean species of the genus Lathyrus belonging to four sections (Lathyrus, Clymenum, Nissolia andAphaca). The molecular characterization and fingerprint identification of 11 populations representing the Lathyrus specieswere conducted using 11 ISSR primers (seven 3’ anchored primers and four non-anchored primers). A total of 261 polymorphicmarkers have been successfully generated, ranging between 400 and 3000 base pairs, with a mean of23.72 bands/primer. The percentage of polymorphic bands for these species was 98.12%. The ISSR technique was ableto characterize and differentiate between the different studied genotypes of the Lathyrus species. Genetic distance withinand between populations was estimated by calculating the similarity index. The similarity indices ranged from 0.251 to0.741, with a mean of 0.386. Our data provide evidence of high molecular polymorphism, showing that both wild andcultivated species forms constitute an important p